Effect of Neuraminidase on the Rat One-Way Mixed Lymphocyte Interaction

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Abstract
The effect of Vibrio cholerae neuraminidase (VCN) on the rat one-way mixed leukocyte interaction (MLI) was investigated. VCN was iodinated without apparent loss of biological activity. Treatment of stimulatory cells with either cold or 125I-labeled VCN significantly enhanced their subsequent reactivity in the MLI. In contrast, treatment of the responding cells alone did not increase the response. Our studies rule out the possibility that VCN increases cellular immunogenicity by binding to the lymphocyte surface. The mechanism of VCN action appears to involve a direct surface modification of lymphoid cells; this modification leads to their increased reactivity.