Enzymatic synthesis of 5-bromo-2’-deoxyuridine-2-14C and of 5-iodo-2’-deoxyuridine-2-14C and their incorporation into deoxyribonucleic acid (Allium cepa)
- 1 September 1964
- journal article
- research article
- Published by Institute of Experimental Botany in Biologia plantarum
- Vol. 6 (3) , 232-235
- https://doi.org/10.1007/bf02926683
Abstract
5-Bromo-2’-deoxyuridine-2-14C was prepared from 5-bromouracil-2-14C and 2’-de-oxyguanosine using trans-N-deoxyribosylase fromLactobacillus helveticus and incorporated into DNA ofAllium cepa roots. After isolating the DNA and hydrolyzing it enzymatically to deoxynucleoside-5’-phosphates a radioactive nucleotide was detected which yielded 5-bromo-2’-deoxyuridine-2-14C on enzymatic dephosphorylation. The incorporation of 5-iodo-2’-deoxy-uridine-2-14C was followed only by microautoradiography.Keywords
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