White Blood Cell Differentials as Performed by the Technicon H•1®: Evaluation and Implementation in a Tertiary Care Hospital

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Abstract
The performance of the Tecbnicon H•1® was evaluated in a tertiary care hospital. More than 500 samples, including those from 150 healthy subjects, were selected over a 1-week period and run on the H•1 and by reference methods. The study was performed following the guidelines outlined in the National Committee for Clinical Laboratory Standards Leukocyte Differential Counting H20-T Tentative Standard, with minor revisions, to determine the clinical sensitivity of the H•1. Correlation studies yielded excellent data for both the complete blood count (CBC) and differential cell count, and all significant morphological abnormalities were reliably flagged. Guidelines for use of the H•1 differential were implemented, and after more than 1 year of online usage, a retrospective study was undertaken to evaluate the effect of the H•1 differential on the laboratory workload. Of the 975 differential counts that were requested by physicians, 60.1 % were reported from the H•1 without any further white blood cell (WBC) review, 14.7% required only a WB C morphology scan, 19.1% had a complete manual differential done, and 6.1% had an insufficient number of cells for a routine differential count. The H•1, therefore, is a suitable instrument for providing both CBCs and differential counts and has made a significant impact on the laboratory work flow.

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