Binding of Metal Ions to Melanin and Their Effects on the Aerobic Reactivity¶
- 1 November 2004
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 80 (3) , 477-481
- https://doi.org/10.1111/j.1751-1097.2004.tb00117.x
Abstract
The binding of Mg(II), Ca(II), Zn(II), Cu(II) and Fe(III) to ETDA‐washed Sepia melanin is quantified by inductively coupled plasma mass spectrometry. By monitoring the solution pH change associated with metal uptake, it is concluded that Mg(II), Ca(II) and Zn(II) bind to carboxylic acid groups in melanin, Cu(II) binds to hydroxyl (OH) groups and Fe(III) binds to OH or amine groups. The aerobic reactivity of melanins with different metal contents is analyzed by examining their ability to cause strand breaks in super‐coiled pUC18 DNA. Cu(II)‐ and Fe(III)‐enriched melanins induce the most damage. Hydroxyl radical, *OH, is proposed to be one of the reactive oxygen species responsible.Keywords
This publication has 16 references indexed in Scilit:
- Characterization of the Fe(III)‐binding Site in Sepia Eumelanin by Resonance Raman Confocal Microspectroscopy¶Photochemistry and Photobiology, 2004
- Ion‐Exchange and Adsorption of Fe(III) by Sepia MelaninPigment Cell Research, 2004
- Characterization of the Fe(III)-binding Site in Sepia Eumelanin by Resonance Raman Confocal Microspectroscopy¶Photochemistry and Photobiology, 2004
- The role of transition metals in the pathogenesis of Parkinson's diseaseJournal of the Neurological Sciences, 1995
- The effect of a synthetic neuromelanin on yield of free hydroxyl radicals generated in model systemsBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1995
- RESISTANCE OF PIGMENTED HUMAN CELLS TO KILLING BY SUNLIGHT AND OXYGEN RADICALSPhotochemistry and Photobiology, 1987
- Free radicals from eumelanins: Quantum yields and wavelength dependenceArchives of Biochemistry and Biophysics, 1984
- PHOTOCHEMISTRY OF PHEOMELANIN: ACTION SPECTRUM FOR SUPEROXIDE PRODUCTIONPhotochemistry and Photobiology, 1980
- Photoreactions of melanin: A new transient species and evidence for triplet state involvementBiochemical and Biophysical Research Communications, 1979