Undifferentiated F9 embryonal carcinoma cells produce a short-chain collagen molecule

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Abstract
The undifferentiated F9 embryonal carcinoma cells produce a unique collagen that decreases in amount during retinoiic acid-induced differntiation of F9 cells into basement-membrane parietal endoderm. A bacterial-collagenase-sensitive protein of approx. 60,000 Da was resolved on polyacrylamide-gel electrophoresis. After pepsin digestion, two pepsin-resistant fragments contianing hydroxyproline were demonstrated, suggesting that a portion of the molecule has a stable triple helix. The mRNA from the undifferentiated F9 cells translates a collagenase-sensitive protein with a molecular mass consistent with the 60,000 Da collagenosu protein produced by undifferentiated F8 cells.