The importance of glutathione and glutathione transferase for somatic mutations in Drosophila melanogaster induced in vivo by 1,2-dichloroethane
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 11 (8) , 1399-1402
- https://doi.org/10.1093/carcin/11.8.1399
Abstract
Two principal pathways of metabolism of the carcinogenic compound 1,2-dichloroethane (DCE) have been proposed. One is a mixed function oxidase dependent pathway requiring oxygen and NADPH. The other pathway depends on the presence of glutathione (GSH) and glutathione transferase (GST). The aim of this study was to investigate the role of the latter pathway for the in vivo mutagenicity of DCE in the somatic wing spot test in Drosophila melanogaster. DCE caused a dose-dependent increase of wing spots. In order to investigate the role of cellular GSH for the mutagenicity, the level of GSH was decreased by 24 h pretreatment with buthionine sulfoximine (BSO), an efficient inhibitor of GSH synthesis. This pretreatment decreased the GSH level to .apprx. 6% as compared to the control. The pretreatment also resulted in a significant decrease of the mutagenicity of DCE. Treatment of the larvae with phenobarbiturate (PB) resulted in .apprx. 200% induction of cytosolic GST, and a corresponding increase in the DCE mutagenicity. These results indicate that the important pathway in vivo for the mutagenicity of DCE is dependent on GSH and GST. A similar experimental protocol was used to study interactions between aflatoxin B1 (AFB) and GSH and GST. No effect of the treatment with BSO on the mutagenicity of AFB was observed, while pretreatment with PB caused a decrease of the mutagenicity of AFB.This publication has 16 references indexed in Scilit:
- Potent and specific inhibition of glutathione synthesis by buthionine sulfoximine (S-n-butyl homocysteine sulfoximine).Published by Elsevier ,2021
- In vitro activation of 1,2-dichloroethane by microsomal and cytosolic enzymesToxicology and Applied Pharmacology, 1980
- The mutagenic effect of 1,2-dichloroethane on Salmonella typhimurium. II. activation by the isolated perfused rat liverChemico-Biological Interactions, 1979
- INVITRO METABOLISM OF 1,2-DIHALOETHANES TO ETHYLENE1979
- ELEVATION OF EXTRA-HEPATIC GLUTATHIONE S-TRANSFERASE AND EPOXIDE HYDRATASE ACTIVITIES BY 2(3)-TERT-BUTYL-4-HYDROXYANISOLE1979
- Mutagenicity studies with X-ray-contrast media, analgesics, antipyretics, antirheumatics and some other pharmaceutical drugs in bacterial, Drosophila and mammalian test systemsMutation Research/Genetic Toxicology, 1979
- ELEVATION OF HEPATIC GLUTATHIONE S-TRANSFERASE ACTIVITIES AND PROTECTION AGAINST MUTAGENIC METABOLITES OF BENZO(A)PYRENE BY DIETARY ANTIOXIDANTS1978
- Mutagenic effects of petrol in Drosophila melanogaster I. Effects of benzene and 1,2-dichloroethaneMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1978
- The mutagenic effect of 1,2-dichloroethane on Salmonella typhimurium I. Activation through conjugation with glutathion in vitroChemico-Biological Interactions, 1977
- A simplification of the protein assay method of Lowry et al. which is more generally applicableAnalytical Biochemistry, 1977