Mannanase Man26A fromCellulomonas fimihas a mannan-binding module
Open Access
- 1 February 2000
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 183 (2) , 265-269
- https://doi.org/10.1111/j.1574-6968.2000.tb08969.x
Abstract
A modular mannanase (Man26A) from the bacterium Cellulomonas fimi contains a mannan-binding module (Man26Abm) that binds to soluble but not to insoluble mannans. Man26Abm does not bind to cellulose, chitin or xylan. The Kd for binding of Man26Abm to locust bean gum (LBG) is ∼0.2 μM. Man26A is the first mannanase reported to contain a mannan-binding module.Keywords
This publication has 19 references indexed in Scilit:
- Enzymatic activity and β-galactomannan binding property of β-mannosidase from Trichoderm reeseiEnzyme and Microbial Technology, 1999
- β-1,4-Glycanases of Cellulomonas fimi: Families, Mechanisms, and KineticsPublished by American Chemical Society (ACS) ,1996
- MICROBIAL HYDROLYSIS OF POLYSACCHARIDESAnnual Review of Microbiology, 1996
- Degradation of cellulases in cultures ofCellulomonas fimiFEMS Microbiology Letters, 1996
- Interaction of Polysaccharides with the N-Terminal Cellulose-Binding Domain ofCellulomonas fimiCenC. 1. Binding Specificity and Calorimetric AnalysisBiochemistry, 1996
- The fine structures of carob and guar galactomannansCarbohydrate Research, 1985
- Affinity electrophoresis: Principles and applicationsElectrophoresis, 1984
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Carbohydrate determination with 4-hydroxybenzoic acid hydrazide (PAHBAH): Effect of bismuth on the reactionAnalytical Biochemistry, 1977
- A new reaction for colorimetric determination of carbohydratesAnalytical Biochemistry, 1972