The Presence of the Adrenergic Nenrotransmitter in Intraaxonal Structures.

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Abstract
Summary.: In cell‐free homogenates of adrenergic nerves in 0.3–0.88 M sucrose, a sediment rich in noradrenaline can be obtained by high speed centrifugation. An active sediment was also obtained by centrifugation of press juice from adrenergic nerves. Noradrenaline was concentrated up to 21 times in the sediment as compared with the original nerve tissue. The highest proportion of noradrenaline recovered in the sediment was 37 per cent of the total activity. While little or no activity was released by suspending the sediment in 0.3 M sucrose, addition of acid to pH 4 or lower released all, and heating most of the activity. Acidification, heating, or addition of detergents followed by freezing and thawing increased the free noradrenaline in the supernatant before, but not after, high speed centrifugation. Histamine was present in the supernatant but not in the sediment containing the noradrenaline. Noradrenaline was demonstrated by column chromatography of extracts of the sediment but no other catechols. It is concluded that noradrenaline is present in high concentration in disperse structures in the adrenergic axones.

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