A New Staining Technique for Ketosteroid Chromatograms

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Abstract
A staining technique is described in which 17-ketosteroids on paper chromatograms undergo complete reaction when immersed for a prolonged period. An aqueous, alkaline, m-dinitrobenzene reagent was the reaction medium used for this purpose. Steroids were intensely stained while background reagent was removed by water washes. Quantification was provided by elution of the Zimmermann reactive chromogens with dimethyl sulfoxide, followed by spectrophotometric measurement of the eluate. Linear and reproducible calibration lines were found with solvent elution of the dyed zones. Densitometric scanning can also be used to yield reasonable quantitative results.

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