Structure elucidation of sulphated oligosaccharides from recombinant human tissue plasminogen activator expressed in mouse epithelial cells
- 1 October 1992
- journal article
- research article
- Published by Oxford University Press (OUP) in Glycobiology
- Vol. 2 (5) , 411-418
- https://doi.org/10.1093/glycob/2.5.411
Abstract
Sulphated N-linked carbohydrate chains isolated from recombinant human tissue plasminogen activator expressed in mouse epithelial (C127) cells were analysed as oligosaccharide alditols by methylation analysis, liquid secondary ion mass spectrometry, and one- and two-dimensional 1H-NMR spectroscopy. The results demonstrate that the major component has the following novel structure: NeuAcα2–6 Galβ1–4 GlcNAcβ1–2 [NeuAcα2–3 Galβ1–4GlcNAcβ1–4]-Manα1–3[NeuAcα2–3(SO4-6)Galβ1–4GlcNAcβ1–2Manα1–6]-Manα1–4GlcNAcβ1–4[Fucα1–6]GlcNAc-ol.Keywords
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