Direct Staining of Reticular Fibers with Gold Chloride

1 January 1962

journal article
research article
Published by Taylor & Francis in Stain Technology

Vol. 37 (1) , 31-34
https://doi.org/10.3109/10520296209114566

Abstract

Reticular fibers are selectively stained in paraffin sections of formalin-fixed or Bouin's-fixed tissue as follows: 1% aqueous solution of gold chloride for 20 min, followed by a 10 min immersion in an aqueous solution containing 5% Na₂CO₃ and 0.5% KOH. The sections then are placed in a 5% aqueous solution of KI for 2 min. Counterstaining with a 0.25% aqueous solution of methylene blue chloride is optional. The reticular fibers stain dark pink; the collagen bundles are a light pink to straw color without the counterstain, or a light blue color when the methylene blue is used.

Keywords

This publication has 3 references indexed in Scilit:

Selective Demonstration of Elastin, Reticulum and Collagen by Silver, Orcein and Aniline Blue
Stain Technology, 1960
Histological and Histochemical Uses of Periodic Acid
Stain Technology, 1948
The Nerve Supply of the Dentine
Proceedings of the Royal Society of Medicine, 1912