Localization of the amino acid substitution site in a fast migrating variant of human serum albumin
- 21 January 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 180 (1) , 85-88
- https://doi.org/10.1016/0014-5793(85)80237-4
Abstract
Albumin Mi/Fg is an Italian genetic variant of human serum albumin arising from a Lys → Glu substitution which has been located in a CNBr fragment (CNBr VII) corresponding to the ‐COOH terminal portion of the molecule [(1984) J. Chromatogr. 298, 336‐344]. Tryptic peptides of CNBr VII from normal and Mi/Fg albumin have been purified by reverse‐phase high‐performance liquid chromatography (RP‐HPLC) and submitted to comparative structural studies. The amino acid sequence of the tryptic peptide of Mi/Fg variant that differs from the corresponding fragment of the normal serum albumin shows that the Lys → Glu substitution responsible for this variant is located at postion 573. This region of the albumin molecule is involved in the binding of long chain fatty acids.Keywords
This publication has 12 references indexed in Scilit:
- Separation of cyanogen bromide fragments from normal and abnormal human serum albumin by reversed-phase high-performance liquid chromatographyJournal of Chromatography A, 1984
- Nucleotide sequence and the encoded amino acids of human serum albumin mRNA.Proceedings of the National Academy of Sciences, 1982
- Localization of the amino acid substitution site in a new variant of human serum albumin, albumin Mexico-2Proceedings of the National Academy of Sciences, 1980
- Genetic and Drug-Induced Variation in Serum AlbuminPublished by Elsevier ,1980
- Human serum albumin. Spectroscopic studies of binding and proximity relationships for fatty acids and bilirubin.Journal of Biological Chemistry, 1979
- A homozygote for a serum albumin variant of the fast typeHuman Genetics, 1979
- Human serum albumin: twenty‐three genetic variants and their population distributionAnnals of Human Genetics, 1973
- Amino acid substitution in two identical inherited human serum albumin variants. Albumin Oliphant and albumin Ann ArborBiochemistry, 1972
- [26a] Sequence analysis with dansyl chloridePublished by Elsevier ,1972
- Strategy and tactics in protein chemistryBiochemical Journal, 1970