“Microcanals” for micropipette access to single cells in microfluidic environments
- 1 January 2004
- journal article
- Published by Royal Society of Chemistry (RSC) in Lab on a Chip
- Vol. 4 (5) , 420-424
- https://doi.org/10.1039/b404956j
Abstract
We demonstrate the fabrication and operation of “microcanals” (i.e. open-air microfluidic channels without a roof), which enable micropipette manipulation and probing of cells within a microfluidic environment. The microcanal devices are fabricated in PDMS on glass substrates using a PDMS membrane transferring technique. Here we show patch-clamp electrophysiological recording and intracellular dye injection performed on cells seeded in microcanals.Keywords
This publication has 49 references indexed in Scilit:
- Micropatterns of Chemisorbed Cell Adhesion-Repellent Films Using Oxygen Plasma Etching and Elastomeric MasksLangmuir, 2003
- Physical, mechanical, and biocompatibility evaluation of three different types of silicone rubberJournal of Applied Polymer Science, 2003
- Activity of single ion channel proteins detected with a planar microstructureApplied Physics Letters, 2002
- Neutrophil chemotaxis in linear and complex gradients of interleukin-8 formed in a microfabricated deviceNature Biotechnology, 2002
- Differential measurement with a microfluidic device for the highly selective continuous measurement of histamine released from rat basophilic leukemia cells (RBL-2H3)Lab on a Chip, 2002
- Micromachined impedance spectroscopy flow cytometer for cell analysis and particle sizingLab on a Chip, 2001
- Hemocompatibility, biocompatibility, inflammatory and in vivo studies of primary reference materials low‐density polyethylene and polydimethylsiloxane: A reviewJournal of Biomedical Materials Research, 2001
- Spatiotemporal protein crystal growth studies using microfluidic silicon devicesJournal of Crystal Growth, 1999
- Cellular Micropatterns on Biocompatible MaterialsBiotechnology Progress, 1998
- A Novel Instrument for Studying the Flow Behaviour of Erythrocytes through Microchannels Simulating Human Blood CapillariesMicrovascular Research, 1997