Primary structure determination and cloning of the cDNA encoding toxin 4 of the scorpion Centruroides noxius Hoffmann
- 29 March 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 320 (1) , 43-46
- https://doi.org/10.1016/0014-5793(93)81654-i
Abstract
A peptide (toxin II‐10), shown to be a Na+ channel blocker, was purified from the venom of the scorpion Centruroides noxius Hoffmann and sequenced by Edman degradation. It has 66 amino acid residues with the C‐terminal residue (asparagine) amidated, as demonstrated by mass spectrometry. In addition, we report the cloning and the nucleotide sequence of the cDNA (CngtV) that codes for this toxin. We discuss the mechanism for processing the precursor peptide to its final form and compare the primary structure to that of other Na− channel toxins. Two distinct groups of toxins seem to emerge from this comparison, suggesting a structure—function relationship of these peptides towards the recognition of either mammalian or insect tissues.Keywords
This publication has 11 references indexed in Scilit:
- Molecular cloning and nucleotide sequence analysis of a cDNA encoding the main β‐neurotoxin from the venom of the South American scorpion Tityus serrulatusFEBS Letters, 1992
- Amino acid sequence and immunological characterization with monoclonal antibodies of two toxins from the venom of the scorpion Centruroides noxius HoffmannEuropean Journal of Biochemistry, 1992
- Nucleotide sequence and structure analysis of a cDNA encoding an alpha insect toxin from the scorpion Leiurus quinquestriatus hebraeusToxicon, 1991
- Characterization of the Actions of Toxins II‐9.2.2 and II‐10 from the Venom of the Scorpion Centruroides noxius on Transmitter Release from Mouse Brain SynaptosomesJournal of Neurochemistry, 1987
- Interactions of Scorpion Toxins with the Sodium ChannelAnnals of the New York Academy of Sciences, 1986
- Scorpion toxins from Centruroides noxius and Tityus serrulatus. Primary structures and sequence comparison by metric analysisBiochemical Journal, 1985
- Mechanism of C-terminal amide formation by pituitary enzymesNature, 1982
- Selective blockage of voltage-dependent K+ channels by a novel scorpion toxinNature, 1982
- The amino terminal sequence of several toxins from the venom of the Mexican scorpion Centruroides noxius HoffmannCarlsberg Research Communications, 1981
- A Protein SequenatorEuropean Journal of Biochemistry, 1967