THE EXTRACTION OF WHEAT STRIATE MOSAIC VIRUS FROM DISEASED WHEAT PLANTS

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Abstract
Extracts from wheat plants infected with wheat striate mosiac virus were shown to be infective by injection into virus-free leafhoppers (Endria inimica Say). To prepare the infective extracts, diseased plants were homogenized in phosphate-buffered saline with or without magnesium chloride and glycine, heated at 45 °C for 10 minutes, and concentrated by differential centrifugation. The virus was protected by the addition of magnesium chloride and glycine. Most of the virus was sedimented at 78,400 g for 60 minutes but a decrease in viral activity occurred during centrifugation.