Selective Monitoring of Stern-Volmer Quenching of Chiral Molecules Via Fluorescence Detected Circular Dichroism
- 1 May 1987
- journal article
- spectrometry
- Published by Taylor & Francis in Analytical Letters
- Vol. 20 (5) , 717-730
- https://doi.org/10.1080/00032718708062923
Abstract
The use of fluorescence detected circular dichroism (FDCD) for monitoring the quenching of chiral molecules is described. This technique combines the specificity for chiral centers of circular dichroism (CD), the sensitivity and selectivity of the fluorescence measurement with the additional chemical information provided by fluorescence quenching. Spectra are acquired using a multidimensional FDCD spectrometer which obtains a FDCD matrix of spectral data as a function of multiple excitation wavelengths (CD information) and multiple fluorescence emission wavelengths. This combination of FDCD and fluorescence quenching produces a very selective monitor for chiral fluorophores.Keywords
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