Abstract
Nuclear Overhauser effect one-dimensional and two-dimensional nuclear magnetic resonance experiments are reported for prostaglandin E1. The two-dimensional nuclear Overhauser effect measurements are combined with spin-echo correlated spectroscopy in order to assign the complete high-field proton magnetic resonance spectrum of 6-keto-prostaglandin E1. The advantages of these two-dimensional techniques are illustrated in these studies. It is also observed that the 6-keto-prostaglandin E1 H-10α proton is substituted more readily by deuterium than are the other protons α to the keto groups.