Biosynthesis of Active Bacillus subtilis Urease in the Absence of Known Urease Accessory Proteins
Open Access
- 15 October 2005
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 187 (20) , 7150-7154
- https://doi.org/10.1128/jb.187.20.7150-7154.2005
Abstract
Bacillus subtilis contains urease structural genes but lacks the accessory genes typically required for GTP-dependent incorporation of nickel. Nevertheless, B. subtilis was shown to possess a functional urease, and the recombinant enzyme conferred low levels of nickel-dependent activity to Escherichia coli . Additional investigations of the system lead to the suggestion that B. subtilis may use unidentified accessory proteins for in vivo urease activation.Keywords
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