Effects of 60 Co Gamma Irradiation on the Polymerization of Microtubules in Vitro

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Abstract
Microtubules [from calf brains] depolymerized in vitro into a high MW aggregate and a 6S component. Irradiation of these components separately indicated that the 6S component was preferentially damaged. This was shown by a reduction in the extent of polymerization following reconstitution of the irradiated and control fractions. The irradiation had no apparent effect on either protein kinase activity or on 14C-colchicine binding ability. Irradiation of depolymerized microtubules at various protein concentrations demonstrated that the 6S tubulin was also preferentially damaged in the presence of the high MW component.

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