Carbohydrate recognition by Clostridium difficile toxin A
- 16 April 2006
- journal article
- research article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 13 (5) , 460-461
- https://doi.org/10.1038/nsmb1084
Abstract
Clostridium difficile TcdA is a large toxin that binds carbohydrates on intestinal epithelial cells. A 2-Å resolution cocrystal structure reveals two molecules of α-Gal-(1,3)-β-Gal-(1,4)-β-GlcNAcO(CH2)8CO2CH3 binding in an extended conformation to TcdA. Residues forming key contacts with the trisaccharides are conserved in all seven putative binding sites in TcdA, suggesting a mode of multivalent binding that may be exploited for the rational design of novel therapeutics.Keywords
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