CLEAVAGE OF CELLOTRIOSE BY MYROTHECIUM CELLULASE
- 1 January 1956
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 34 (1) , 83-89
- https://doi.org/10.1139/o56-011
Abstract
The method of cleavage of cellotriose by Myrothecium cellulase was determined from the distribution of C14in the enzymic hydrolysis products of cellotriose labelled in the anhydroglucose unit at the reducing end of the chain. The substrate was prepared by the incubation of the enzyme in a concentrated solution of cellopentaose (0.14 M) and C14-labelled glucose (5 M) and the fractionation of the products, first by adsorption and elution on a charcoal column and then by preparative paper chromatography. The glucosidic linkage adjacent to the non-reducing end of the chain is preferentially hydrolyzed by the enzyme. Its rate of hydrolysis was estimated to be about five times that for the linkage adjacent to the reducing end of the chain.Keywords
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