A HISTOCHEMICAL METHOD FOR THE DEMONSTRATION OF AMINOPEPTIDASE B ACTIVITY
Open Access
- 1 March 1972
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 20 (3) , 192-194
- https://doi.org/10.1177/20.3.192
Abstract
A histochemical method for the demonstration of aminopeptidase B activity is presented. The method is based on the specific activation of the enzyme by chloride ions present in physiologic concentrations (0.9% or 0.154 M) in the incubating medium, on the inhibition by phosphate ions and on the use of N-l-arginyl-2-naphthylamine as substrate. The liberated 2-naphthylamine was coupled with fast blue B.Keywords
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