Phosphorylation of Ribosomal Proteins Induced by Auxins in Maize Embryonic Tissues
Open Access
- 1 November 1990
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 94 (3) , 1270-1275
- https://doi.org/10.1104/pp.94.3.1270
Abstract
The effect of auxin on ribosomal protein phosphorylation of germinating maize (Zea mays) tissues was investigated. Two-dimensional gel electrophoresis and autoradiography of [32P] ribosomal protein patterns for natural and synthetic auxin-treated tissues were performed. Both the rate of 32P incorporation and the electrophoretic patterns were dependent on 32P pulse length, suggesting that active protein phosphorylation-dephosphorylation occurred in small and large subunit proteins, in control as well as in auxin-treated tissues. The effect of ribosomal protein phosphorylation on in vitro translation was tested. Measurements of poly(U) translation rates as a function of ribosome concentration provided apparent Km values significantly different for auxin-treated and nontreated tissues. These findings suggest that auxin might exert some kind of translational control by regulating the phosphorylated status of ribosomal proteins.Keywords
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