Differential β‐arrestin binding of AT1and AT2angiotensin receptors

Abstract
Agonist stimulation of G protein‐coupled receptors causes receptor activation, phosphorylation, β‐arrestin binding and receptor internalization. Angiotensin II (AngII) causes rapid internalization of the AT1receptors, whereas AngII‐bound AT2receptors do not internalize. Although the activation of the rat AT1Areceptor with AngII causes translocation of β‐arrestin2 to the receptor, no association of this molecule with the AT2receptor can be detected after AngII treatment with confocal microscopy or bioluminescence resonance energy transfer. These data demonstrate that the two subtypes of angiotensin receptors have different mechanisms of regulation.