Immunohistochemical detection of infectious bursal disease and infectious bronchitis viral antigens in fixed, paraffin‐embedded chicken tissues

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Abstract
Immunoperoxidase and immunofluorescent techniques were used to detect and localise infectious bursal disease virus (IBDV) and infectious bronchitis virus (IBV) in fixed, paraffin‐embedded chicken tissues. A modified Bouin's solution (9 parts of 1% picric acid to 1 part of 10% neutral buffered formalin and 5% acetic acid) proved to be the fixative of choice. Tissues fixed in 10% neutral buffered formalin showed heavy background staining, whereas enzyme treatment of these tissues succeeded in exposing viral antigens and thus giving a specific reaction. For routine diagnostic purposes it will prove advantageous to detect IBDV and IBV in fixed, paraffin‐embedded material.