Biochemical evidence that phytochrome of the moss Ceratodon purpureus is a light‐regulated protein kinase
- 2 January 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 315 (1) , 69-73
- https://doi.org/10.1016/0014-5793(93)81135-m
Abstract
The phytochrome gene of the moss Ceratodon purpureus (phyCer) codes for a novel phytochrome polypeptide with a predicted molecular mass of 145 kDa that has a COOH‐terminal domain which is homologous to the catalytic domain of eukaryotic protein kinases. In this paper we report the first biochemical evidence that in fact, as predicted from the gene sequence, PhyCer represents an active, light‐regulated protein kinase. In vitro phosphorylation experiments with protonemata extracts revealed the existence of a 140 kDa protein, phosphorylated in a red/far‐red light dependent manner. The binding of a polyclonal antibody directed to the protein kinase catalytic domain of PhyCer enhanced the phosphorylation of a 140 kDa band when assayed in a renaturation‐auto‐phosphorylation experiment with nitrocellulose bound protein. These findings strongly implicate that the phyCer gene product has protein kinase activity and is capable of auto‐phosphorylation. The results of the renaturation‐phosphorylation experiments were essentially the same, no matter whether protein extracts from light grown or dark adapted moss protonemata were used. Thus, phyCer expression most likely is not light regulated.Keywords
This publication has 24 references indexed in Scilit:
- Molecular identification of a soybean protein kinase gene family by using PCRPlant Molecular Biology, 1992
- Nucleotide sequence of a cDNA encoding a protein kinase homologue inArbidopsis thalianaPlant Molecular Biology, 1992
- PHYTOCHROME: A Light-activated Molecular Switch that Regulates Plant Gene ExpressionAnnual Review of Genetics, 1991
- Protein Phosphorylation in Green Plant ChloroplastsAnnual Review of Plant Biology, 1991
- [1] Protein kinase classificationPublished by Elsevier ,1991
- Characterization and Distribution of a Maize cDNA Encoding a Peptide Similar to the Catalytic Region of Second Messenger Dependent Protein KinasesPlant Physiology, 1990
- Phytochrome in lower plants Detection and partial sequence of a phytochrome gene in the moss Ceratodon purpureus using the polymerase chain reactionFEBS Letters, 1990
- Molecular cloning of plant transcripts encoding protein kinase homologs.Proceedings of the National Academy of Sciences, 1989
- Regulation of enzyme activity in plants by reversible phosphorylationPhysiologia Plantarum, 1988
- Phosphorylation of Proteins in Plants: Regulatory Effects and Potential Involvement in Stimulus/Response CouplingAnnual Review of Plant Physiology, 1987