Identification of inhibitors of nitric oxide synthase that do not interact with the endothelial cell l‐arginine transporter

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Abstract
The effects of inhibitors of nitric oxide (NO) synthase and other cationic amino acids on unidirectional l-arginine transport were studied in porcine aortic endothelial cells cultured in microwell plates or perfused in microcarrier columns. l-Homoarginine, l-lysine and l-ornithine inhibited transport of l-arginine. The NO synthase inhibitors NG-monomethyl-l-arginine and NG-iminoethyl-l-ornithine also reduced l-arginine uptake, whereas NG-nitro-l-arginine and its methyl-ester had no inhibitory effect. The ability to modulate selectively endothelial cell l-arginine transport or NO synthase activity will allow further characterization of the arginine transporter and its role in regulating NO biosynthesis.

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