RELEASE OF HEPATITIS A VIRUS INTO THE CULTURE-MEDIUM DURING ITS PROPAGATION IN PLC/PRF/5 CELLS

  • 1 January 1983
    • journal article
    • research article
    • Vol. 297  (3) , 111-114
Abstract
Hepatitis A virus (HAV), obtained from human feces collected 3 days after the onset of jaundice, was propagated in human hepatocarcinoma cells (PLC/PRF/5). In the course of 5 passages, it was released into the supernatant without cytopathic effect. Released HAV was propagated after inoculation into PLC/PRF/5 cells. Its biophysical properties (buoyant density in CsCl; 1.33 g/cm3; sedimentation coefficient: 155 S) were similar to those of the infectious particle present in the cells or in the stools of patients.

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