Increased Rate of Repair of Ultraviolet-induced DNA Strand Breaks in Mitogen Stimulated Lymphocytes
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine
- Vol. 41 (5) , 483-491
- https://doi.org/10.1080/09553008214550561
Abstract
The rate of repair of strand breaks in UV-irradiated bovine lymphocytes was studied. The extent of breakage of DNA was the same in mitogen-stimulated and unstimulated lymphocytes from 2 breeds of cattle, when determined by sedimentation of nucleoids on sucrose gradients. In mitogen-stimulated cells the time taken to repair DNA strand breaks was 6 h compared to 12 h in stationary phase lymphocytes after a UV dose of 5 J/m2. Apparently, the increased rate of repair of strand breaks is due to the induction of enzymes involved at the post-incision stage of DNA repair. Thus, the increased level of repair synthesis observed in earlier work correlates with an increased rate of repair of DNA strand breaks in phytohemagglutinin-stimulated bovine lymphocytes.This publication has 17 references indexed in Scilit:
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