Use of glnQ as a Counterselectable Marker for Creation of Allelic Exchange Mutations in Group B Streptococci
- 1 January 2005
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 71 (1) , 587-90
- https://doi.org/10.1128/aem.71.1.587-590.2005
Abstract
Efficient allelic exchange mutagenesis in group B streptococci (GBS) has been hampered by the lack of a counterselectable marker system. Growth inhibition of GBS by the glutamine analog gamma-glutamyl hydrazide requires glnQ . We have used this phenomenon to create a counterselectable marker system for efficient selection of allelic exchange mutants in GBS.Keywords
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