A Protein, VPg, Covalently Linked to 36S Calicivirus RNA

1 March 1980

journal article
Published by Microbiology Society in Journal of General Virology

Vol. 47 (1) , 215-220
https://doi.org/10.1099/0022-1317-47-1-215

Abstract

Proteins associated with 36S virus RNA from Vero cells infected with San Miguel sea lion virus, type 2 (SMSV-2), were labelled with ¹²⁵I. One protein, VPg, remained linked to RNA when subjected to deproteinization techniques. VPg labelled with ³²P was observed on 36S RNA from purified virions; the quantity of label was compatible with two phosphates per genome. The estimated mol. wt. of SMSV-2 VPg was 15000.

Keywords

VPG
VIRIONS
VERO CELLS
PHOSPHATES
COVALENTLY LINKED
CALICIVIRUS RNA
36S CALICIVIRUS
SMSV

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