Dicer is required for chromosome segregation and gene silencing in fission yeast cells
- 13 December 2002
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 99 (26) , 16648-16653
- https://doi.org/10.1073/pnas.212633199
Abstract
RNA interference is a form of gene silencing in which the nuclease Dicer cleaves double-stranded RNA into small interfering RNAs. Here we report a role for Dicer in chromosome segregation of fission yeast. Deletion of the Dicer (dcr1+) gene caused slow growth, sensitivity to thiabendazole, lagging chromosomes during anaphase, and abrogated silencing of centromeric repeats. As Dicer in other species, Dcr1p degraded double-stranded RNA into ≈23 nucleotide fragments in vitro, and dcr1Δ cells were partially rescued by expression of human Dicer, indicating evolutionarily conserved functions. Expression profiling demonstrated that dcr1+ was required for silencing of two genes containing a conserved motif.Keywords
This publication has 42 references indexed in Scilit:
- El Niño Events Recorded by Stalagmite Carbon IsotopesScience, 2002
- The genome sequence of Schizosaccharomyces pombeNature, 2002
- Requirement of Heterochromatin for Cohesion at CentromeresScience, 2001
- An Abundant Class of Tiny RNAs with Probable Regulatory Roles in Caenorhabditis elegansScience, 2001
- Identification of Novel Genes Coding for Small Expressed RNAsScience, 2001
- A Role for the RNase III Enzyme DCR-1 in RNA Interference and Germ Line Development in Caenorhabditis elegansScience, 2001
- A Cellular Function for the RNA-Interference Enzyme Dicer in the Maturation of thelet-7Small Temporal RNAScience, 2001
- Genes and Mechanisms Related to RNA Interference Regulate Expression of the Small Temporal RNAs that Control C. elegans Developmental TimingCell, 2001
- RNAiCell, 2000
- Position effect variegation at fission yeast centromeresCell, 1994