A Multimycotoxin Detection Method for Aflatoxins, Ochratoxins, Zearalenone, Sterigmatocystin, and Patulin

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Abstract
In response to the need for a more comprehensive and simple procedure for multiple mycotoxin detection than had heretofore been deyised, a method has been developed for detection of aflatoxins B1, B2, G1, and G2, ochratoxins A and B and their ethyl esters, zearalenone, sterigmatocystin, and patulin. The method is based on selective extraction with acetonitrile-water, defatting with isooctane, and removal of water-soluble components by transfer of the mycotoxins to chloroform. The method has been applied to corn, barley, oats, and wheat. The detection limits achieved are not as low as can be attained with the analytical procedures for the individual mycotoxins, but are low enough to be useful in a screening procedure.

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