Activities of liver mitochondrial and peroxisomal fatty acid oxidation enzymes in rats fedtrans fat

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Abstract
The effect oftrans fat on the activities of liver mitochondrial and peroxisomal fatty acid oxidation enzymes was examined in various strains of rats. When Wistar and Sprague-Dawley rats were fed for 30 days diets containing either olive oil or partially hydrogenated corn oil as a source ofcis-ortrans-octadecenoate, respectively, the activities of various enzymes of mitochondrial and peroxisomal β-oxidation measured withcis- andtrans-9-octadecenoic acid as substratese showed little dietary fatdependent change. In Fischer 344 rats, feedingtrans fat for 15 mo increased only moderately various enzymes of β-oxidation except for carnitine acyltransferase. The rate of mitochondrial ketogenesis and the activity of carnitine acyltransferase measured withtrans-9-octadecenoic acid as a substrate were about half those with thecis-counterpart. Peroxisomes oxidizedtrans-9-octadecenoyl-CoA at a rate comparable to thecis-counterpart. It was concluded from this study and previous ones that the difference in the geometry of dietary fatty acid had only a marginal effect in modulating the hepatic fatty acid oxidation system, in spite of marked differences in the metabolic behavior ofcis-andtrans fatty acid in cell-free preparations and perfused liver.