Relationship between Poly(Adenosine 5′-diphosphate-ribose) Synthesis and Transcriptional Activity in Wheat Embryo Chromatin

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Abstract
Chromatin-bound poly(ADP-ribose) synthetase activity was highest in ungerminated wheat (T. aestivum L., cv. Mukakomugi) embryos and it decreased after germination within 14 h. Transcriptional activity was lowest in ungerminated wheat embryos and it increased during germination for 24 h or more. Histones, H1, H2A/H2B, basic nonhistone chromosomal proteins and acidic nonhistone chromosomal proteins (MW > 10 kdaltons) were ADP-ribosylated in wheat germ chromatin. Specific nonhistone chromosomal protein (MW of 37 kdaltons) in seedling chromatin was not ADP-ribosylated.
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