Serum Effect on the Killing of Staphylococcus Aureus by Human Leukocytic Extracts

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Abstract
Summary: The killing phenomenon of Staphylococcus aureus by human blood leukocytes is reconstituted extracellularly. The greatest killing action is manifested with the extract of the lysosomal granules of the leukocyte lysate around pH 5.0. Humoral factors as provided by the serum of healthy human adults significantly increase the rate of killing of the staphylococci. The effectiveness of human serum in preparing the bacteria for extracellular bactericidal action is considerably reduced by heating at 56°C, by adsorption with aggregated human γ-globulin or by the addition of ethylenediaminetetracetic acid in the serum.