Identification of monohydroxylated metabolites of cannabidiol formed by rat liver

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Abstract
Cannabidiol (CBD) was metabolized in vitro by rat liver enzymes. Unchanged CBD and eight monohydroxylated metabolites were isolated and positively identified. As previously reported, 7‐hydroxy‐CBD was the major metabolite. The second most abundant metabolite was 6α‐hydroxy‐CBD; whereas only a trace amount of 6β‐hydroxy‐CBD was found. In addition hydroxylation occurred in all positions of the pentyl side chain, 4″‐hydroxy‐CBD being most abundant. 3″‐Hydroxy‐CBD was formed in half of the yield of 4″‐hydroxy‐CBD, while 1″‐, 2″‐, and 5″‐hydroxy‐CBD were each formed in approximately one fourth of the yield of 4″‐hydroxy‐CBD.