Tissue‐specific binding of a nuclear factor to the insulin gene promoter
Open Access
- 5 October 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 222 (2) , 332-336
- https://doi.org/10.1016/0014-5793(87)80396-4
Abstract
Using a DNA‐binding gel mobility shift assay, a protein‐DNA complex was detected using the rat II insulin promoter and a nuclear extract from rat insulinoma cells. This complex was detected using extracts from other insulin‐producing cell lines but was not found using nuclear extracts from non‐insulin‐producing pancreatic cell lines or other cell types. Binding of the tissue‐specific protein to the labelled promoter fragment was effectively competed by both rat and human insulin genes but not by the SV40 TATA box. This protein‐DNA interaction may be involved in tissue‐specific regulation of insulin gene expression.Keywords
This publication has 24 references indexed in Scilit:
- Cloned cell lines from a transplantable islet cell tumor are heterogeneous and express cholecystokinin in addition to islet hormones.The Journal of cell biology, 1986
- Synergism between immunoglobulin enhancers and promotersNature, 1986
- Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box regionCell, 1985
- Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitroCell, 1985
- Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteinsNature, 1985
- Heritable formation of pancreatic β-cell tumours in transgenic mice expressing recombinant insulin/simian virus 40 oncogenesNature, 1985
- Enhancers and eukaryotic gene transcriptionTrends in Genetics, 1985
- Correct transcription of an immunoglobulin κ gene requires an upstream fragment containing conserved sequence elementsNature, 1984
- A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosomeCell, 1984
- Cell-specific expression controlled by the 5′-flanking region of insulin and chymotrypsin genesNature, 1983