Gas Chromatographic Determination of Fluridone Aquatic Herbicide and Its Major Metabolite in Fish

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Abstract
A gas-liquid chromatographic (GLC) method is described for determining residues of the aquatic herbicide fluridone (l-methyl-3-phenyl-5-[3-(trifluoromethyl)phenyl]-4(lH)-pyridinone) and its major metabolite (l-methyl-3-(4-hydroxypherayl)-5-[3-(trifluoromethyl)phenyl]-4(lH)-pyridinone) in fish. Both compounds are extracted from fish tissue with methanol, and the extracts are subjected to acidic hydrolysis to release conjugated forms of fluridone and the metabolite. After purification by liquid-liquid partitioning, sample extracts are reacted with methyl iodide to methylate the metabolite, and then both fluridone and the metabolite are brominated with phosphorus tribromide. After purification by Florisil column chromatography, the derivatives are separated and measured by electron capture GLC. The method is capable of determining approximately 0.01 ppm of both compounds in fish, and recoveries have averaged 84±14.7% for fluridone and 83±13.4% far the metabolite.

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