Effects of Purine Derivatives and Analogues on Multiplication of the Vaccinia Virus

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Abstract
Summary: 2,6-diaminopurine inhibits multiplication of the vaccinia virus in an in vitro system containing chick embryonic tissue. This inhibition is abolished by adenine at an equivalent concentration, and by adenylic and guanylic acids, yeast nucleic acid, diphosphopyridine nucleotide and hypoxanthine at somewhat higher concentrations. Guanine, xanthine and uracil are inactive. Several halogenated purines appear to be virostatic in the same system, but adenine did not prevent the inhibition of viral growth produced by 2,6,8-trichloropurine. Three triazolopyrimidines, including 8-azaguanine, failed to prevent multiplication of the virus. Benzimidazole is inhibitory but the toxicity is not abolished by adenine, guanine or yeast nucleic acid. Mice inoculated intracerebrally with a neurotropic strain of vaccinia virus (IHD) were not protected by 2,6-diaminopurine, 2,6-dichloro-7-methyl purine or benzimidazole.