Synthesis of cholesterol‐20‐14C

Abstract
Cholesterol‐20‐14C was synthesized in four steps from pregnenolone‐20‐14C. The labelled intermediates were proven to be cholest‐5‐ene‐3β, 20α‐diol acetate‐3, cholesta‐5, 20 (22)‐diene‐3β‐ol acetate, and cholesterol acetate by 1H‐n.m.r. spectroscopical analysis. Cholesterol‐20‐14C was obtained with a radiochemical purity exceeding 99%.