Synthesis of oltgonucleotides with sequences identical with or analogous to the 3′-end of 16S ribosomal RNA of Es cherichia coli: preparation of m62A-C-C-U-C-C and A-C-C-U-C-m42C via phosphotriester intermediates1
- 1 January 1977
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (3) , 747-759
- https://doi.org/10.1093/nar/4.3.747
Abstract
The synthesis of two fully-protected hexanucleotides (11a and 11b) via a phosphotriester approach, which is based on the use of two types of protecting groups for the internucleotide linkages, i.e. one 2,2,2-tribromo-ethyl at the 5'-terminus and four 2-chlorophenyl groups for the remaining linkages, is reported. The hexanucleotides 11a and 11b, assembled via a block-wise two-step phosphotriester method, can be deblocked conveniently to give the two hexamers 12a and 12b containing only 3'leads to5' internucleotide linkages.Keywords
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