Determination of Atenolol Combinations with Hydrochlorothiazide and Chlorthalidone in Tablet Formulations by Reverse-Phase HPLC

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Abstract
A high performance liquid chromatographic procedure is presented for the simultaneous determination of atenolol, hydrochlorothiazide, and chlorthalidone in pharmaceutical tablets combinations. An aliquot of the sample is dissolved in methanol containing methyl p-hydroxybenzoate as an internal standard and chromatographed on a supelcosil (5 um) LC-8-DB (250mm × 4.6mm i.d.) column using a mobile phase of 1.0 mm ammonium acetate and 2.0 mm octanesul fonic acid sodium salt in acetonitrile: water (25 : 75) solution, the pH was adjusted to 3.5 with glacial acetic acid. The relative standard deviations are less than 1 % for the five commercial tablets analyzed. The method was tested for linearity, recovery, and specificity and was found to be fast, stability-indicating, and free from interferences.