POSSIBILITY OF A RAPID METHOD FOR SENSITIVITY TESTING OF MYCOBACTERIA BY DETERMINATION OF URACIL-H-3 INCORPORATION

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 239  (1) , 87-94
Abstract
Dubos-Tween Albumin cultures (8.0 ml) were set up with 106 viable bacteria/ml. After incubation for 48 h 0.25 .mu.Ci H3-uracil/ml was added. At the same time the antibiotic was introduced. The final concentrations were 1 .mu.g, 0.25 .mu.g and 0.025 .mu.g for INH (isonicotinic acid hydrazide); 4.0 .mu.g and 0.4 .mu.g for SM (dihydrostreptomycin); 32 .mu.g and 0.32 .mu.g for RMP (rifampicin); 2.0 .mu.g and 1.0 .mu.g for ETH (Myambutol); 0.5 .mu.g and 0.25 .mu.g for PAS (Pasalon). The incorporation rate clearly depended on the degree of sensitivity of the bacteria. Determining the incorporation rate of H3-uracil allowed the result to be read after only 1 wk instead of 4 to 6 wk using the conventional method on solid media. Turbidity measurements of the cultures gave an equally reliable result enabling this latter method to be performed in less well equipped laboratories.

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