An improved pyrogallol red-molybdate method for determining total urinary protein.
Open Access
- 1 November 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 35 (11) , 2233-2236
- https://doi.org/10.1093/clinchem/35.11.2233
Abstract
We adapted the pyrogallol red-molybdate method for total urinary protein to the Cobas Bio centrifugal analyzer. The method is simple, rapid, sensitive, and inexpensive. Addition of 25 mg of sodium dodecyl sulfate per liter to the reagent modifies protein reactivities so that the chromogenicity of human gamma globulins is the same as that of albumin. Results by this method and a comparison method that included gel filtration and a modified biuret reaction correlated well (r = 0.951).This publication has 10 references indexed in Scilit:
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