Effect of prevention of procollagen triple-helix formation on proline 3-hydroxylation in freshly isolated chick-embryo tendon cells
- 15 April 1981
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 196 (1) , 203-206
- https://doi.org/10.1042/bj1960203
Abstract
Inhibition of procollagen triple-helix formation by the addition of cis-hydroxyproline or azetidine-2-carboxylic acid increased the synthesis of 3-hydroxy[14C]proline 1.7-1.8-fold in pulse-chase experiments with freshly isolated chick embryo tendon cells. The amount of 3-hydroxy[14C]proline, expressed as a percentage of the total 14C radioactivity in hydroxyproline, reached 8.4%. Control experiments indicated that the 2 analogs had no effect on the prolyl 3-hydroxylase activity of these cells. Apparently, the time available before triple-helix formation in part regulates the extent of the 3-hydroxylation of proline in the biosynthesis of collagen in intact cells.This publication has 29 references indexed in Scilit:
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