The Porcine Mammary Gland as a Bioreactor for Complex Proteinsa
- 1 June 1994
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 721 (1) , 218-233
- https://doi.org/10.1111/j.1749-6632.1994.tb47394.x
Abstract
The similar biological activity of rhPC and hPC indicates that porcine mammary gland can perform many of the processing reactions necessary for recombinant synthesis of complex human proteins and produce them at levels suitable for industrial bioreactor applications. The health of the transgenic pigs appeared unaffected by the expression of high levels of the heterologous protein. We suggest that one of the advantages of using the mammary gland as a bioreactor appears to be the high cell density relative to that of cell culture.Keywords
This publication has 30 references indexed in Scilit:
- Production of Biologically Active Human Protein C in the Milk of Transgenic MiceAnnals of the New York Academy of Sciences, 1992
- High Level Expression of Active Human Alpha-1-Antitrypsin in the Milk of Transgenic SheepNature Biotechnology, 1991
- A .gamma.-carboxyglutamic acid (.gamma.) variant (.gamma.6D, .gamma.7D) of human activated protein C displays greatly reduced activity as an anticoagulantBiochemistry, 1990
- Characterization and Novel Purification of Recombinant Human Protein C from Three Mammalian Cell LinesNature Biotechnology, 1990
- Expression of Human Anti-Hemophilic Factor IX in the Milk of Transgenic SheepNature Biotechnology, 1989
- Clonal analysis of the glycosylation of immunoglobulin G secreted by murine hybridomasBiochemistry, 1989
- Protein C prevents the coagulopathic and lethal effects of Escherichia coli infusion in the baboon.Journal of Clinical Investigation, 1987
- A procedure for the complete clarification of milk of various species and its suitability for use with colorimetric measurementsJournal of Dairy Research, 1984
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970