Abscisic Acid and Sucrose Control of Velvetleaf (Abutilon theophrasti) Ovule Development in Vitro

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Abstract
The culture of ovules excised from velvetleaf (Abutilon theophrasti Medic., ♯4 ABUTH) capsules 5 days after anthesis was used to measure the effects of abscisic acid (ABA) and sucrose on embryo development and prevention of precocious germination. ABA at 1 × 10-7 M combined with 6% sucrose in the medium for the first 14 days of culture increased embryo development but prevented precocious germination. Higher concentrations of ABA inhibited embryo development. Without ABA, precocious germination increased directly with the concentration of sucrose in the medium, and embryos died. In vivo, ABA reached its highest concentration in ovules 5 days after anthesis but was undetectable after 16 days. Parental control of embryo development may involve ABA and an increasing concentration of osmoticum as seeds dehydrate during maturation.