The Effects of Cellular Glutathione Elevation on the Oxygen Enhancement Ratio
- 1 August 1985
- journal article
- research article
- Published by JSTOR in Radiation Research
- Vol. 103 (2) , 232-239
- https://doi.org/10.2307/3576577
Abstract
The radiation responses at various O2 tensions were evaluated in V79 Chinese hamster cells under conditions where their nonprotein thiols, primarily glutathione (GSH), were elevated by 2-oxothiazolidine-4-carboxylate (OTZ). OTZ, when cleaved by intracellular oxoprolinase, provides the cell with cysteine which stimulates GSH synthesis. A 2-h pretreatment with 10 mM OTZ elevated GSH to 200% of controls. This elevation in GSH offered no protection to aerated cells. However, for O2 tensions .ltoreq. 40,000 ppm modest protection was observed as evidenced by an increase in the O2 enhancement ratio. GSH elevation afforded maximal protection between 1000 and 10,000 ppm O2. However, the extent of protection was relatively small (protection factor = 1.3).This publication has 11 references indexed in Scilit:
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