pH dependent conformation of physalaemin
- 1 August 1989
- journal article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 34 (2) , 139-147
- https://doi.org/10.1111/j.1399-3011.1989.tb01503.x
Abstract
The undecapeptide physalaemin was investigated by n.m.r. spectroscopy in DMSO solution under acidic and neutral conditions. Large changes of the NH chemical shifts and the temperature gradients of the NH protons occurred on going from pH 3.5 to pH 7.0 for residues around the charged amino acids Asp and Lys. At pH 3.5 the data are in accord with a flexible conformation of the peptide. The results at neutral pH are interpreted in terms of a folded structure having two interresidue and one intraresidue hydrogen bond. They include a beta turn with proline in position i + 1 and asparagine in position i + 2.Keywords
This publication has 35 references indexed in Scilit:
- Stereochemical studies on cyclic peptides. XIII. Energy minimization studies on cyclic hexapeptides having hydrogen bonds*International Journal of Peptide and Protein Research, 1987
- Peptide models of electrostatic interactions in proteins: NMR studies on two .beta.-turn tetrapeptides containing Asp-His and Asp-Lys salt bridgesBiochemistry, 1986
- Conformation analogy between substance P and physalaeminBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1986
- Are the proposed substance P receptor sub-types, substance P receptors?Life Sciences, 1984
- Conformation and Biological Activity of Cyclic PeptidesAngewandte Chemie International Edition in English, 1982
- The tachykinin peptide familyTrends in Neurosciences, 1981
- Investigation of complex networks of spin-spin coupling by two-dimensional NMRJournal of Magnetic Resonance (1969), 1981
- NMR evidence for a type I β‐turn in (Pro2)‐tetrapeptides and interdependence of cis:Trans isomerism, ring flexibility, and backbone conformationBiopolymers, 1980
- β-turns in proteinsJournal of Molecular Biology, 1977
- Nuclear magnetic resonance study of some α-amino acids—II. Rotational isomerismSpectrochimica Acta, 1964